MOHAMMAD ABU DARWISH1, Z.M. ABU-DIEYEH2, AHMAD H. AL-FRAIHAT2, SATI Y. AL-DALAIN2
1Al-Balqa Applied University, Ash-Shoubak University College, Dept. of Applied and Essential Sciences, Jordan
2Al-Balqa Applied University, Ash-Shoubak University College, Dept. of Agricultural Sciences, Jordan
Essential oil yields and heavy metal contents were determined in the sage medicinal plant, Salvia officinalis L., cultivated at 15, 30 and 45cm inter-raw spacing in Ash"=Shoubak in the South of Jordan. Samples were harvested during vegetation (VEG), beginning of blooming (BB), full"=blooming (FB) and fruit maturation (FM) stages. Essential oil yield and the content of heavy metals in the plant were determined by using hydrodistilation and atomic absorption spectrometry methods, respectively. The yields of essential oil and heavy metal contents were affected by inter"=raw spacing and pheniological stage. The maximum oil yield 2.000.115% was obtained in plants cultivated with 15cm inter-raw spacing and harvested at VEG stage, while the minimum (0.87%) was obtained in plants cultivated with the same inter"=raw spacing but harvested at FM stage. Heavy metal contents were variable depending upon both inter"=raw spacing and pheniological stage. Co, Cd, and Pb were not detectable. The content of Ni, Zn, Fe, and Cu were increased during the vegetative life cycle of the plant but still below their toxic level. Salvia officinalis, cultivated in Ash"=Shoubak region is found to be rich in oil extract and free from hazard heavy metals.
Keywords: Essential oil, heavy metals, Jordan, sage, Salvia