SOLMERYHEL RODRÍGUEZ1, GUSTAVO FERMÍN2, MARÍA RAYMÚNDEZ1, ANTONIETA PORCO3, ISELEN TRUJILLO4, RAINER SCHULTZE-KRAFT5, TEODARDO CALLES6
1Central University of Venezuela, Institute for Experimental Biology, Venezuela
2University of the Andes, Mérida Botanical Garden Center, Venezuela
3Simón Bolívar University, Department of Cell Biology, Venezuela
4Simón Rodríguez National University of Technology, Institute for Scientific and Technological Studies, Venezuela
5International Center for Tropical Agriculture (CIAT), Colombia
6Foundation Botanical Institute of Venezuela (FIBV), Venezuela
Centrosema is an economically important Neotropical genus which, together with Desmodium and Stylosanthes, is regarded as a genus with particularly high potential as a forage legume in the tropics and sub-tropics. Several Centrosema species are currently being used not only as forage but also for soil cover and soil improvement. Some species are well adapted to prolonged drought and the acid, low"=fertility soils that prevail in the tropics, thus making Centrosema species particularly important for low"=input agricultural production systems.
There are some uncertainties regarding species delimitation and evolutionary relationships within the genus, due to the morphological plasticity of the species. Consequently, there is a number of differences between species which are not discernable using only morphological analysis. Therefore, the main objective of this research is to assess species delimitation and phylogenetic relationships among Venezuelan Centrosema species, using molecular data, as a complement of morphological studies. Molecular analyses are based on polymorphism of intergenic sequences of cpDNA atp-rbcL and trnK/matK, Internal Transcribed Spacer (ITS) of rDNA, Random Amplification of Polymorphic DNA (RAPD), and Simple Sequence Repeats (SSRs).
Preliminary results of the intergenic sequences trnK/matK (trnK685F/matK1932) and atp-rbcL (R7/S11) show, on the one hand, that Centrosema has a monophyletic origin, i.e., the species have a common ancestor. On the other hand, the grouping of species (clades) obtained by these molecular markers differs, in some cases, from the classification proposed by Williams and Clements (1990) where the Centrosema species were classified in 11 sub-generic groups based on morphological similarities. More conclusive results are expected when further data are obtained.
Keywords: Centrosema, introns, ITS, phylogenetics, RAPD, SSRs