Mingrelia España, Thomas Brune, Ellen Kandeler, Georg Cadisch:
Effect of Plant Residue Quality on Soil Fungal Community in a Vertisol


1University of Hohenheim, Institute of Plant Production and Agroecology in the Tropics and Subtropics, Germany
2University of Hohenheim, Institute for Soil Science and Land Evaluation, Germany

Fungi play a major role in C and N cycling of plant residues, because of their ability to degrade complex substrates. Slow growing saprotrophic fungi are able to degrade lignin using extracellular enzymes and thereby gaining access to protected cellulose and hemi-cellulose compounds. On the other hand, fast growing opportunistic fungi (sugar fungi) are stimulated by easy accessible carbon sources. To evaluate the effect of plant residue quality on soil fungal community, an incubation experiment was conducted with highly 15N-enriched (99 atom%) plant residues of different quality, i.e. maize (C:N= 32, lignin:N=2.2) and soybean (C:N= 15, lignin:N=1.1), incorporated (1%) in a Vertisol soil, taken from a long"=term field experiment carried out in Venezuela since 1997. The residues were incubated for 30 days (25°C) at 40% WHC. A control without residue was also used. The ergosterol content was measured after 3, 7, 15 and 30 days. DNA was extracted and the active and passive fungal community composition is being analysed by using the 15N-DNA stable isotopic probing (SIP) and molecular (DGGE, cloning and sequencing) techniques. Residues additions stimulated soil fungal activities and the quality of residues influenced the microbial biomass. The fungal biomass was higher in the soybean treatment already at early stages of decomposition compared to the maize treatment. This seems to indicate that quality of residues affected the fungal community, i.e. in soybean it is probably composed mainly of sugar fungi and in maize probably dominated by slow growing lignocellulytic fungi. Further DNA analysis should help clarifying this difference. High correlation was found between fungal biomass and enzymes activities involved in C-cycling suggesting that fungi play a major role in the recycling of C and nutrients in the tropical soil.

Keywords: Ergosterol, fungal community, residue quality, vertisol


Contact Address: Mingrelia Espaņa, University of Hohenheim, Institute of Plant Production and Agroecology in the Tropics and SubtropicsGarbenstr. 13, 70593 Stuttgart, Germany, e-mail: mespana@uni-hohenheim.de
Andreas Deininger, November 2007