Sarawut Boonpeng, Suchada Vearasilp, Pimchai Apavatjrut, Sangtiwa Suriyong, Elke Pawelzik:
Effect of Storage Temperature and Dehydration Induction Rates to Germeability of Sugarcane Synthetic Seeds

SARAWUT BOONPENG$1$, SUCHADA VEARASILP$1$, PIMCHAI APAVATJRUT$2$, SANGTIWA SURIYONG$1$, ELKE PAWELZIK$3$
$1$Chiang Mai University, Department of Agronomy, Thailand
$2$Chiang Mai University, Department of Horticulture, Thailand
$3$Georg-August-Universität Göttingen, Institute for Agricultural Chemistry, Germany

Effect of storage temperature and dehydration induction rates on germeability of sugarcane synthetic seeds were investigated. Compact and white sugarcane (Saccharum officinarum L.) embryogenic callus developed from young leaf-rolled cultured on Murashige and Skoong agar medium supplemented with 3mg/l 2,4-D, 50mg/l cystein and 5% coconut water under dark condition at 25°C. Initiation of embryogenic cell suspension achieved in liquid MS basal medium supplemented with 3mg/l 2,4-D, 10% coconut water and 400mg/l casein hydrolysate and kept in continuous dark at 25°C. The medium was replaced with fresh medium every 4 days interval to reduced accumulation of phenolic compound. Somatic embryos developed after 6 weeks when transferred embryogenic cell to fresh medium without 2,4-D under 16-h photoperiod. Mature somatic embryos were encapsulated in 3 percent sodium alginate for synthetic seeds production. Sugarcane synthetic seeds were stored in 3 different temperatures (4+1, 15+2 and 25+2°C) under 16-h photoperiod for 4 weeks. It was found that synthetic seeds stored at 4+1°C for 4 weeks showed no precoccius germination or death during storage and could be germinated at 35 percent. A hydrated synthetic seeds retained enough water for the encapsulated somatic embryos to germinated during storation. However, germination was blocked when hydrated alginate capsules were dehydrated. It was found that desiccated synthetic seeds with siliga gel until 80 percent water loss showed no precoccius germination and could be germinated at 27 percent after stored for 4 weeks. Therefore, storing hydrated sugarcane synthetic seeds in 4+1°C and dehydration with silica gel until 80 percent water loss, which might be especially useful for prevent precocious germination during short term sugarcane synthetic seeds storage.

Keywords: Hydrolysate, phenolic compound, somatic embryo, synthetic seed

Footnotes

... 

Contact Address: Sangtiwa Suriyong, Chiang Mai University, Department of Agronomy, Huay Kaew Road, 50200 Chiang Mai, Thailand, e-mail: sangtiwachiangmai.ac.th