Nurhayati, Grete Thinggaard, Udo ter Meulen:
Accumulation of 15 N in Yolks and Albumen of Hens Fed Diets Containing 15 N-CCC During Egg Development


1 University of Jambi, Department of Animal Nutrition and Feed Science, Indonesia
2 Georg-August-Universität Göttingen, Institute of Animal Physiology and Nutrition, Germany

An experiment was conducted to evaluate the accumulation of dietary 15 N-CCC in yolks and albumen of hens fed diets containing 15 N-CCC during egg development. Twenty 280 days old Brown breed layer hens were fed a 15 N-CCC free balanced diet with 16.37% crude protein and 11.48 MJ ME/kg. In completely randomised design, the hens were divided into 4 groups (A,B,C and D) and given on one of the 4 treatments: 0 mg 15 N-CCC/kg feed (control diet/group A), 5 mg 15 N-CCC/kg feed (group B), 50 mg 15 N-CCC/kg feed (group C) and 100 ppm mg 15 N-CCC/kg feed (group D) for 11 days. During the 7 days followed, 15 N-CCC treatments were withdrawn and all chickens restored to feeding on the control diet. Eggs were collected daily during both periods and egg yolks and albumen were separated. The 15 N content was measured using a coupled Elemental Analyser-Continuous Flow II Interface-Isotope Ratio Mass Spectrometer (EA-ConFloII Interface-IRMS) and the δ15 N excess ( δ15 N-ex) and atom percentage 15 N (At%) calculated. There was no significant (p > 0.05 ) difference in δ15 N-ex and At% of egg yolks and albumen of group B both during 11 days of feeding on 15 N-CCC containing diets and during the 7 days 15 N" CCC diets withdrawal. Feeding with 15 N" CCC affected (p < 0.05 ) δ15 N-ex and At% in egg yolks and albumen of group C and D from 8 and 3 days respectively after the beginning of feeding 15 N-CCC diets, and up to 2 and 4 days after 15 N" CCC diets were withdrawn, respectively. The δ15 N-ex and At% in egg yolks tended to be higher than in albumen during the treatment period. The 15 N concentrations reduced after 15 N-CCC diets were withdrawn. These results suggest that hens might transfer excess dietary 15 N-CCC or its metabolites into eggs and 15 N accumulates during egg development. However, by this method it is not possible to determine if 15 N is still bound in CCC or in its metabolite products.

Keywords: Atom percentage 15 N, chlorocholine chloride, δ15 N, egg


Contact Address: Udo ter Meulen, Georg-August-Universität Göttingen, Institute of Animal Physiology and Nutrition, Kellnerweg 6, 37077 Göttingen, Germany, e-mail:
Andreas Deininger, 2003