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Tropentag, September 14 - 16, 2022, Prague

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Metabolomics of Vernonia amygdalina Del. (Compositae) chaff supplemented feed

Ipinamo Joshua Ogoina1, Helen Ajayi2, Ruth Tariebi S. Ofongo3

1Niger Delta University, Animal Science
2Benson Idahosa University, Animal Science, Nigeria
3Niger Delta University, Dept. of Animal Science, Nigeria


Vernonia amygdalina Del (Compositae) is an herbaceous shrub with medicinal properties. Its phytochemical constituents are known for their biological and physiological benefits when consumed by both man and animals. A core benefit of metabolomics analysis is that it gives the totality of chemical compounds present within a system such as biological fluids, plants (fruit or vegetables) and processed food products. This study was designed to determine the secondary metabolites present in feed of laying hens supplemented with V. amygdalina chaff.
Clean fresh leaves of V. amygdalina – bitter leaf was obtained air dried and milled in an electric grinding machine to obtain a powdery form. The finely ground leaves were supplemented at 50 g/kgDM of complete feed into a commercial layer’s diet to feed laying hens. One hundred gram of the leaf supplemented feed was taken to the laboratory for metabolomic analysis using gas chromatography-mass spectroscopy (GCMS). Prior to GCMS analysis, finely ground feed sample was extracted using methanol as extraction solvent being an untargeted metabolomic analysis. The analysis was performed using 7820A gas chromatograph coupled to 5975C inert mass spectrometer (with triple axis detector) and electron impact source (Agilent Technologies). Helium was used as carrier gas at a constant flow rate of 1.573 ml/min, with an initial nominal pressure of 1.9514 psi and at an average velocity of 46 cm/s. One microliter of the samples were injected in splitless mode at an injection temperature of 260°C. The MS was operated in electron‐impact ionisation mode at 70eV with ion source temperature of 230°C, quadruple temperature of 150°C and transfer line temperature of 280°C. Scanning of possible compounds was from m/z 30 to 550 amu at 2.62s/scan scan rate. Compounds present were identified by comparing measured mass spectral data with those in NIST 14 Mass Spectral Library. Hexadecanoic acid methyl ester (C16H32O2), Dodecanoic acid (C12H24O2), 9,12, Octadecanoic acid methyl esther E.E. and 9, Octadecanoic acid methyl esther were detected in feed sample examined.
These metabolites have potential biological and physiological benefits as components of livestock feed.

Keywords: Feed, metabolomics, secondary metabolites, Vernonia amygdalina

Contact Address: Ruth Tariebi S. Ofongo, Niger Delta University, Dept. of Animal Science, Wilberforce Island, P.M.B. 071, Yenagoa, Nigeria, e-mail: tariruth@live.de

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