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Tropentag, September 15 - 17, 2021, hybrid conference

"Towards shifting paradigms in agriculture for a healthy and sustainable future"

Fortifying Fermenting Enset (Ensete ventricosum) with Moringa Leave and Garden Cress: Physicochemical and Microbial Profiling

Addisu Fekadu Andeta1, Fantahun W. Misganaw2, Gemechu Leta Debele1, Ribka Ebrahim3

1Arba Minch University, Microbial and Molecular Systems, Ethiopia
2Ethiopian Biotechnology Institute, Industrial Biotechnology Directorate, Ethiopia
3Arba Minch University, Inst. of Technology, Dept. of Water supply and Env’t. Eng., Ethiopia


Enset (Ensete ventricosum) is an important food security crop for over 20 million Ethiopian people after fermentation into kocho. The fermented food product is a good source of starch (65%) and soluble sugars (9%), but it is low in protein (< 4%) and fat (< 1%). The aim of this study was to assess the physicochemical and microbial dynamics during fortified-enset fermentation. To this end, six enset plants were processed using enset processing machines and fortified each with 3% shed-dried Moringa stenopetala leaf powder (MS), Moringa oleifera (MO) leave powder, Lepidium sativum (LS) seed powder and the fourth treatments left as a Control (C). The fortified and control enset samples were allowed to ferment for two months in a Sauerkraut jars. Samples were taken on days 1, 7, 15, 30 and 60. The pH and moisture content were measured. Also, microbial dynamics were determined using the plate count method. The pH of the enset sample fortified with MS, MO, LS and C samples on day 0 were 5.99, 6.15, 5.83 and 6.63, respectively. On day 60 the pH reduced to 4.25, 4.00, 4.28 and 3.92 for MS, MO, LS and C samples, respectively. The total aerobic count attained a maximum of 9.23, 9.40,9.29 and 9.28 log cfu/g at day 0 for MS, MO, LS and C, respectively. Lactic acid bacteria count (LAB) were 8.10, 8.13, 8.10 and 8.10 log cfu/g on day 1. On day 60 the LAB count reduced to 7.34, 7.05, 7.73 and 6.70 for MS, MO, LS and C, respectively. Moreover, enset fortified with MS and MO leave powder inhibited the growth of undesirable microorganisms (Clostridium sp, Enterobacteriaceae and molds) from enset samples on day 15 and onwards. Addition of moringa leaves powder (MS and MO) to fermenting enset showed a significant improvement in the protein, calcium, iron, phosphorus and magnesium contents of kocho. In conclusion, addition of moringa leave powder to fermenting enset significantly decreased Clostridium, yeast and mold counts and Enterobacteriaceae) abundance, whereas it increased Lactobacillus abundance as compared with the untreated enset samples.

Keywords: Enset, fermentation, fortification, Moringa, lactic acid bacteria

Contact Address: Addisu Fekadu Andeta, Arba Minch University, Microbial and Molecular Systems, Secha Condominium, T4-3-11, 4400 Arba Minch, Ethiopia, e-mail: addisu.fekadu@amu.edu.et

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