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Characteristics of PBA Profiling Markers in the Analysis of Arachis hypogaea L. Genome

Julio Montero-Torres1, Michal Droppa2, Tania Pozzo3, Roberto Acebey-Aldunate1, Eloy Fernández Cusimamani4, Jana Žiarovská2

1Universidad Mayor, Real y Pontificia de San Francisco Xavier de Chuquisaca, Fac. of Agricultural Sciences, Bolivia
2Slovak University of Agriculture, Dept. of Genetics and Plant Breeding, Slovakia
3University of California, Dept. of Plant Sciences, United States
4Czech University of Life Sciences Prague, Fac. Trop. AgriSci., Dept. of Crop Sci. and Agroforestry, Czech Republic


Abstract


In this study, the PBA technique was utilised to characterise its effectivity to be used for the possible analysis of Arachis hypogaea L. genome polymorphism. Three different peanut genotypes were chosen that were characterised previously to have different profiles in iPBS fingerprints. All the genotypes were collected from Chuquisaca Department, Bolivia as original plant sources. The seeds were transferred to the Faculty of Tropical Agrisciences, CULS in Prague, Czech Republic and planted in pots. Young plants were transferred to the AgroBioTech Research Centre; SUA in Nitra, Slovak Republic where biological material was analysed. Three different PBA primer combinations were used in PCRs with the result of generating different PBA fingerprint profiles – CYP1A, CYP2B, and CYP2C. A total of 83 amplicons were generated for the analysed peanut accessions with the highest number of 33 amplicons for marker CYP2C, but for this marker, the lowest percentage of polymorphism was obtained on the level of 60 %. CYP1A marker achieved the polymorphism on the level of 63 % and CYP2B marker on the level of 79 %. CYP1A marker achieved the value of effective number of alleles 1.7634 and the Shannon's Information index 0.6245. CYP2B marker achieved the value of effective number of alleles 1.6500 and the Shannon's Information index 0.5830. CYP2C marker achieved the value of effective number of alleles 1.9780 and the Shannon's Information index 0.6876. None of the markers used in this study has generated the same profile for any of the analysed peanut accessions, that is why all of them should be useful for DNA based profiling of Arachis hypogaea L. germplasm, but CYP2B should be used preferably.


Keywords: Arachis hypogaea, genome, germplasm, PBA markers, polymorphism


Contact Address: Jana Žiarovská, University of Agriculture in Nitra, Dept. of Genetics and Plant Breeding, Nitra, Slovakia, e-mail: jana.ziarovska@uniag.sk


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