Genetic diversity of Sesbania sesban collection using DArTseq markers
Alemayehu T. Negawo1, Meki Shehabu Muktar1, Habib Olumide Akinmade 1, Alieu Sartie2, Chris S. Jones3
1International Livestock Research Institute (ILRI), Feed and Forage Development, Ethiopia
Sesbania sesban is a fast-growing short-lived perennial forage crop in the Leguminosae family and is grown primarily for fodder and forage in the tropical and subtropical regions of the world. A collection of Sesbania sesban held in the forage Genebank at the International Livestock Research Institute (ILRI) were studied using genome-wide markers generated on DArTseq platform. Three thousand and forty-two plants from 171 accessions, collected from diverse geographic origins, were genotyped, and the genotyping produced 84,673 SNP and 60,626 SilicoDArT markers with a mean polymorphic information content of 0.153 and 0.123, respectively. The fragment length of the markers’ ranged from 26 to 69 bases with a mean of 66 bases. Around 85.73 % of the SNP and 81.11% of SilicoDArT markers had a fragment length of 69 bases. The hierarchical cluster and DAPC analyses using selected informatic markers (PIC≥0.2) showed the presence of four main clusters in the collection. The hierarchical clustering using SNP and SilicoDArT markers had a cophenetic correlation coefficient of 76.5 %. The partitioning of the total genetic variation into ‘within’ and ‘among’ accessions’ variations was analysed using analysis of molecular variance (AMOVA) and the result showed the ‘within’ accessions’ variation (65.7 % for SNPs and 72.6% for SilicoDArT markers) contributed a larger proportion of the total genetic variation compared to the ‘among’ accessions’ variation. In general, in this study, we generated a large set of genotyping data on Sesbania sesban collection and the generated information could be used for further genomic studies in this important multipurpose forage crop.
Keywords: DArTseq markers, forage, genebank, genetic diversity, Sesbania sesban
Contact Address: Chris S. Jones, International Livestock Research Institute (ILRI), Nairobi, Kenya, e-mail: c.jonescgiar.org