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Tropentag, September 14 - 16, 2022, Prague

"Can agroecological farming feed the world? Farmers' and academia's views."


Antimicrobial activities and phytochemical screening of leaves and fruits of the Cassia fistula L.

Rabha musa Kdam1, Atif abdelmoneim Yassin2, Hasseeb Elbukhary3

1West kordufan university, Faculty of animal production
2Gezira university, Chemical engineering
3West Kordofan University, Faculty of Animal Production, Sudan


Abstract


The Cassia fistula L. tree is widely used in traditional medicinal system and the plant parts have been reported to possess biological activities. The aim of the present study is to investigate the antimicrobial activities and phytochemical screening of leaves and fruits of the C. fistula. This study was conducted at the University of Gezira, Sudan, 2019/20. The leaves and fruits of the tree were obtained from Khartoum and Wad Medani cities. Four concentrations (25, 50, 75 and 100 %) of aqueous extract of the leaves and fruits were made by serial dilution from stock solution (50 mg/ 500 ml) in addition to the control. The concentrations were tested against the fungi and bacteria. The parameters measured were radial growth, spore germination and mycelial fresh and dry weight of the fungi as well as inhibition zone and number of colonies of the bacteria. Different standard methods were used for the phytochemical screening to identify the secondary metabolites in the leaves and fruits. Data were subjected to descriptive analysis and analysis of variance. The results showed that the leaf and fruit aqueous extracts had antifungal activities. The leaf aqueous extracts had higher antifungal activities compared to the fruit aqueous extracts and the Aspergillus niger was more sensitive to the extracts compared to the Penicillium digitatum. Also, the extracts had antibacterial activities. The leaf aqueous extracts at low concentrations (25 and 50%) had lower antibacterial activities compared to the fruit aqueous extracts. However, at the high concentrations (75 and 100%) it had higher antibacterial activities compared to the fruit aqueous extracts. The Escherichia coli was more sensitive to the leaf and fruit aqueous extracts compared to the Staphylococcus aureus. The tannin was detected in leaves and fruits aqueous extracts. Alkaloids and sterols were detected in the leaves only. Saponin and flavonoids were detected in the fruits, but not detected in leaves. In conclusion the aqueous extracts of the leaves and fruits of C. fistula could be used as antifungal and antibacterial agents. Further studies towards isolation and formulation of the active compounds were recommended.


Keywords: Antimicrobial activities, cassia fistula L. tree, traditional medicinal


Contact Address: Hasseeb Elbukhary, West Kordofan University, Faculty of Animal Production, Eljamaa, 11111 El Khawie, Sudan, e-mail: elbukhary11@yahoo.com


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