Tropentag 2023, September 20 - 22, Berlin, Germany "Competing pathways for equitable food systems transformation: trade-offs and synergies."

Body composition changes of male mice C57BL/6 fed high-fat diets supplemented with bovine milk coming from three feeding systems in subhumid tropics

¹Instituto Nacional de Ciencias Médicas y Nutricíon 'Salvador Zubirán', Mexico
²Universidad Nacional Autónoma de México (UNAM), Fac. de Medicina Veterinaria y Zootecnia, Mexico
³Universidad Nacional Autónoma de México (UNAM), Dept. de Ciencias Pecuarias, Mexico

Abstract

The objective of this study was to compare the body weight, fat mass and lean mass in a mouse model fed with a high-fat diet (HFD) supplemented with lyophilized bovine milk coming from three feeding systems from sub-humid tropical of Yucatán, Mexico. The murine model consisted in 30 male C57BL/6 mice of 21 days of age and body weight of 24g. Mice were allocated into 5 groups (n=6). The experimental groups were: 1) CD= control diet (7% fat); 2) HFD= high fat diet (21% fat); 3) ISS= HFD plus 40% lyophilized bovine milk from intensive silvopastoral system; 4) MS= HFD plus 40% lyophilized bovine milk from monoculture system, and 5) NVS= HFD plus 40% lyophilized bovine milk from native vegetation system. The rodents were fed ad libitum during 98 days; body weight and food intake were recorded once a week. Body composition (lean and fat mass) was evaluated in the day 77 by quantitative magnetic resonance (EchoMRI-700 TM; Echo Medical Systems, LLC. Houston, TX, USA). Data were analysed by one-way ANOVA and Tukey (α=0.05). HFD (36.8±3.5g) increased body weight (P=0.0248) compared to CD (29.4±2.9g) and ISS (30.5±1.9g). However, all groups supplemented with cow's milk showed a tendency to lower body weight at greater extent than HFD. For lean or muscle mass, HFD obtained the lowest percentage (68.2±2.4g), being statistically different (P=0.0206) to CD which showed the largest value (77.9±6.4g). No further differences were observed in the rest of groups. Even though, a rising trend was depicted from NVS (70.1%) followed by ISS (75.2%) and MS (76.4%). In contrast to lean mass, fat mass percentage was maximum in HFD (29.5%) being different (P=0.0206) from CD (18.8%) and ISS (19.2%). The SM (20.0%) and NVS groups (26.4%) were not distinct from CD in this respect (P=0.9960 and P=0.1902, respectively). Cow's milk, could a non-pharmacologic approach to modulate metabolic alterations encouraged by a high fat diet. Supplementation cow's milk deflect excessive body weight gain while depleting body fat mass deposition.

Keywords: Adipogenesis, body composition, lipotoxicity

Contact Address: Mario Cuchillo Hilario, Instituto Nacional de Ciencias Médicas y Nutricíon 'Salvador Zubirán', Mexico City, Mexico, e-mail: mario.cuchillohincmnsz.mx