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Effect of Lactobacillus rhamnosus C6 inoculation on fermentation quality and rumen degradability of maize cob and hush silage

¹Chiang Mai University, Dept. of Animal and Aquatic Science, Thailand
²National Science and Technology Development Agency, National Center For Genetic Engineering And Biotechnology (BIOTEC), Thailand
³Veterinary, Conservation and Research Section, Animal Management Division, Chiang Mai Night Safari, Thailand

Abstract

The objective of this study were to evaluate effect of Lactobacillus rhamnosus C6 inoculation on fermentation quality and rumen degradability of maize cob and hush silage. L. rhamnosus C6 were isolated from rumen fluid of Thai native cattle. The isolates was grow in nutrient agar (NA) plate at 37°C for 48 hours in anaerobic condition. Maize cob and hush silage were divided 2 groups as control and supplemented with L. rhamnosus C6 with 10⁶ cfu/g. Silage was collected at 21 of fermentation. The fermentation quality was evaluated by pH measurement and VFA analysis. The chemical compositions were analysed by proximate analysis and detergent fiber method was employed to determine fiber compositions. Ruminal degradability was determined using in vitro gas production technique. Ruminal fluid was obtained from 4 rumen fistulated Thai native cattle. Gas production was recorded after incubation at 2, 4, 8, 10, 12, 24, 48, 72 and 96 hr. The pH value of maize cob and hush silage inoculated with L. rhamnosus C6 were lower than control group. Lactic acid concentration was higher in inoculated groups. Maize cob and hush silage inoculated with L. rhamnosus C6 showed significantly lower NDF, cellulose and hemicellulose contents than control group. Moreover, gas production at 48 and 72 hr after incubation was significantly greater in inoculated groups. It could be concluded that maize cob and hush silage inoculated with L. rhamnosus C6 had better quality fermentation than natural fermentation as L. rhamnosus C6 stimulated the fermentation process and accelerated lactic acid production, resulting in a sharp drop in pH and faster ruminal degradability after 48 hr of incubation.

Keywords: Fermentation quality, Lactobacillus rhamnosus C6, maize cob and hush silage, rumen degradability, Thai native cattle

Contact Address: Saowaluck Yammuen-Art, Chiang Mai University, Dept. of Animal and Aquatic Science, 239 Huay Kaew Road, 50200 Mueang, Thailand, e-mail: saowaluck.ycmu.ac.th