Biological control of southern blight (Sclerotium rolfsii L.) on beans (Phaseolus vulgaris L.) with Trichoderma harzianum R. and three bio-preparations as a soil amendment and seed treatment
Yesenia Marisol Guardado Torres, Diana Alexandra Ramírez Segovia
University of El Salvador, Fac. of Agronomic Sciences, El Salvador
This research was conducted in the laboratory and greenhouse of the Department of Plant Protection of the Faculty of Agricultural Sciences of the University of El Salvador, during the months of March to May 2017. The effectiveness of three bio-preparations (lombriabono, bokashi, and mountain microorganisms), an antagonist Trichoderma harzianum (Excalibur Gold®), a relative control (Copper Hydroxide, (Kocide WG ®), and absolute control (no treatment); and two methods of application (soil amendment and seed pelleting), on pathogenic fungus Sclerotium rolfsii L., which causes Southern blight disease in bean cultivation. The assay was conducted under greenhouse conditions with 11 treatments and 5 repetitions, plus a duplicate of the assay. The observations were carried out from planting to the first month, which is the critical stage of infection by the pathogen, inoculating the pathogenic fungus in the sterile substrate, taking emergency data 5 days after planting the bean, and then every 3 days to measure the level of infection. The data collection was done by the presence of signs of the pathogen and symptoms of the disease, the emergence, and infection in seed, and plant infection was evaluated.
Keywords: Phaseolus vulgaris, Sclerotium rolfsii, Trichoderma harzianum
Contact Address: Yesenia Marisol Guardado Torres, University of El Salvador, Fac. of Agronomic Sciences, CP 1502 Antiguo Cuscatlan, El Salvador, e-mail: marisol.guardadotgmail.com