Tropentag, September 20 - 22, 2017 in Bonn "Future Agriculture: Social-ecological transitions and bio-cultural shifts"

Fermentation Quality and Chemical Composition of Napier Pakchong 1 Silage Supplemented with Lactic Acid Bacteria

Chiang Mai University, Dept. of Animal and Aquatic Science, Thailand

Abstract

The objective of this study was to evaluate effect of lactic acid bacteria supplementation on fermentation quality and chemical composition of Napier Pakchong 1 silage. Lactic acid bacteria (LAB) were extracted from corn silage. The isolates was grow in MRS agar plate at 37 °C for 48 hours in anaerobic condition. The effect of LAB supplementation on fermentation quality and chemical composition of Napier Pakchong 1 silage were evaluated using in a completely randomised design. Napier Pakchong 1 Silage was divided into two groups (control and supplemented with LAB with 1×10⁵ cfu/ml). One kilogram of Napier Pakchong 1 grass was supplemented with 10 ml of LAB. Silage was collected at day 14, 21, 28 and 35 of fermentation. Samples were analysed for pH, lactic acid concentration, chemical composition by proximate analysis and detergent method. It was found that pH of Napier Pakchong 1 silage supplemented with LAB was lower than control. Lactic acid concentration was higher in Napier Pakchong 1 silage supplemented with LAB.The crude protein (CP) and acid detergent fibre (ADF) concentrations of Napier Pakchong 1 silage declined with increasing ensiling time. The CP and ether extract (EE) concentration of Napier Pakchong 1 silage supplemented with LAB was higher than the control (8.57 vs 7.98% and 2.5 vs 2.31%) The crude fibre (CF) concentration of Napier Pakchong 1 silage supplemented with LAB was higher than in the control. It can be concluded that LAB supplementation increased the fermentation of Napier Pakchong 1 silage and decreased the nutrient deterioration of Napier Pakchong 1 silage.

Keywords: Chemical composition, fermentation quality, lactic acid bacteria, napier Pakchong 1, silage

Contact Address: Saowaluck Yammuen-Art, Chiang Mai University, Dept. of Animal and Aquatic Science, 239 Huay Kaew Road, 50200 Chiang Mai, Thailand, e-mail: saowaluck.ycmu.ac.th