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\hypersetup{pdftitle={Optimisation Studies of Lipase"=Producing Bacterium Isolated from Cocoa Processing Plant Effluent},pdfauthor={Samuel Adebisi Adedire, Mufutau Kolawole Bakare, Isaac Olusanjo Adewale, Temitope Owoeye},pdfsubject={Tropentag 2016: Abstract},pdfkeywords={Brevundimonas diminuta, cocoa, effluent, lipase},pdfpagemode=None,colorlinks=true}
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\parbox[b]{13.4cm}{\centering \large{\textbf{Tropentag, September 18-21, 2016, Vienna, Austria}}\\[1ex] \Large{``Solidarity in a competing world ---\\fair use of resources''\\[2ex]}}
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\Large{\textbf{Optimisation Studies of Lipase"=Producing Bacterium Isolated from Cocoa Processing Plant Effluent\footnote{\textbf{Contact Address:} Samuel Adebisi Adedire, Obafemi Awolowo University, Microbiology, Ile-Ife, 234~Ile-ife, Nigeria, \mbox{e-mail}: \email{bisidedire2002@yahoo.com}}\\[0.8ex]}}
\normalsize{\textsc{Samuel Adebisi Adedire$^{1}$, Mufutau Kolawole Bakare$^{1}$, Isaac Olusanjo Adewale$^{2}$, Temitope Owoeye$^{3}$}}
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\begin{itemize*}
\item[]{\small{\textit{$^{1}$Obafemi Awolowo University, Microbiology, Nigeria}}}
\item[]{\small{\textit{$^{2}$Obafemi Awolowo University, Biochemistry, Nigeria}}}
\item[]{\small{\textit{$^{3}$International Institute of Tropical Agriculture (IITA), Bioscience, Nigeria}}}
\end{itemize*}
\index[author]{Adedire, Samuel Adebisi}
\index[author]{Bakare, Mufutau Kolawole}
\index[author]{Adewale, Isaac Olusanjo}
\index[author]{Owoeye, Temitope}
\begin{center}
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\textbf{Abstract}
\begin{abstract}
\normalsize{
This study isolated and screened lipase"=producing bacterium from cocoa processing plant effluent for lipase production consequent upon the springing up of cocoa processing companies in southwestern part of Nigeria. This was with a view to exploiting the bacterial candidate for the production of lipase for industrial and biotechnological uses.



Wastewater samples were collected from cocoa processing plant effluent in Ondo, Nigeria. Lipase"=producing bacteria were screened from the effluent. Four bacterial isolates showed lipolytic activities and were assessed both qualitatively (agar plate cultures) using tributyrin agar and quantitatively (liquid cultures) using olive oil as sole carbon and energy source. The bacterium with the highest lipase activity was selected, identified both biochemically and molecularly using 16S rRNA gene sequencing technique. The optimisation of the production medium for maximum production and lipase activity. Lipase activity was assayed by a spectrophotometric method. The absorbance of p-Nitrophenol released was measured at 410 nm. The 16S rRNA gene sequencing confirmed the bacteria with the highest lipase activity to be \textit{Brevundimonas diminuta}. Maximum enzyme production was obtained when the medium was incubated for 72 h (35.85$\pm$ 0.5\,{U}\,{ml}\textsuperscript{-1}) at temperature of 35°C and maintained at pH 7.5. Olive oil (with 30.00 $\pm$ 4.09\,U\,ml\textsuperscript{-1}) and peptone (with 47.33 $\pm$ 1.26\,{U}\,{ml}\textsuperscript{-1}) were found to be the most suitable substrate for maximum enzyme production. The organism also utilised the effluent (with 4.78 $\pm$ 0.16\,{U}\,{ml}\textsuperscript{-1}) as carbon source. The study concluded that cocoa processing plant effluent contain substrate which encourages the isolation of Lipase"=producing bacteria and also the effluent could serve as alternative source of carbon source for lipase production. 



}

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\noindent \textbf{Keywords:} \textit{Brevundimonas diminuta}, cocoa, effluent, lipase
\index[key]{Brevundimonas diminuta@\textit{Brevundimonas diminuta}}
\index[key]{Cocoa}
\index[key]{Lipase}
\end{document}
