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\hypersetup{pdftitle={Semen Characteristics and Recovery Rate of  Aceh Bull Frozen Semen},pdfauthor={Iis Arifiantini, Marlene Nalley, Eros Sukmawati},pdfsubject={Tropentag 2016: Abstract},pdfkeywords={Aceh cattle, recovery rate , semen characteristic},pdfpagemode=None,colorlinks=true}
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\parbox[b]{13.4cm}{\centering \large{\textbf{Tropentag, September 18-21, 2016, Vienna, Austria}}\\[1ex] \Large{``Solidarity in a competing world ---\\fair use of resources''\\[2ex]}}
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\Large{\textbf{Semen Characteristics and Recovery Rate of  Aceh Bull Frozen Semen\footnote{\textbf{Contact Address:} Iis Arifiantini, Bogor Agricultural University, Dept. of Veterinary Clinic, Reproduction and Pathology, Bogor, Indonesia, \mbox{e-mail}: \email{iis.arifiantinipurna@gmail.com}}\\[0.8ex]}}
\normalsize{\textsc{Iis Arifiantini$^{1}$, Marlene Nalley$^{2}$, Eros Sukmawati$^{3}$}}
\end{center}
\begin{itemize*}
\item[]{\small{\textit{$^{1}$Bogor Agricultural University, Dept. of Veterinary Clinic, Reproduction and Pathology, Indonesia}}}
\item[]{\small{\textit{$^{2}$University of Nusa Cendana, Animal Reproduction, Indonesia}}}
\item[]{\small{\textit{$^{3}$Artificial Insemination Centre, Indonesia}}}
\end{itemize*}
\index[author]{Arifiantini, Iis}
\index[author]{Nalley, Marlene}
\index[author]{Sukmawati, Eros}
\begin{center}
\vspace{1.1cm}
\textbf{Abstract}
\begin{abstract}
\normalsize{
Aceh cattle is an indigenous breed of  Aceh province, the most western province of Indonesia. To improve the genetic quality of Aceh cattle, current breeding using artificial insemination (AI) technology, and the frozen semen of this breed is available at Lembang AI centre. This study were aimed to evaluate the semen characteristic of Aceh breed and to study the spermatozoa recovery rate after freezing. Nine Aceh bulls belong to Nasional Lembang AI centre were used for this research, in total 593 ejaculate during 2015. The semen was collected using artificial vagina and subsequently evaluate macro- and microscopycally according to AI centre procedure. Semen were diluted  with  skim milk eggyolk extender, packed into 0.25 ml straws  and than equilibrate at cooling cabinet and finnaly freezed using automatic freezing machine for 10 minutes. The result demonstrated that colour and aspect of the ejaculates ranged from milky white to creamy, the average of  semen volume was 5.57 $\pm$ 1.66 mL, pH was 6.56 $\pm$0.14. The average of sperm progressive motility and sperm concentration were 67.21$\pm$\mbox{0.09\,\%} and 975.24 $\pm$ 381.72 $\times$ 10\textsuperscript{6} sperm respectively. The average straw produced per ejaculate was varies from 190.14 $\pm$ 66.51 to 380.41 $\pm$ 106.82 straws. The recovery rate were 60.44 $\pm$ 5.42 to 66.01 $\pm$ \mbox{7.41\,\%}, these results were higher than other local breed such as Bali, Madura or Ongole crossbreed. In conclusion, we found wide variability in the sperm concentration among individual Aceh bull as well as in the number of straw produced by each bull and also the value of recovery rate.



}

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\end{abstract}
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\noindent \textbf{Keywords:} Aceh cattle, recovery rate , semen characteristic
\index[key]{Aceh cattle}
\index[key]{Recovery rate }
\index[key]{Semen characteristic}
\end{document}
