\documentclass[english,german]{scrartcl}
\usepackage[latin1]{inputenc}
\usepackage{geometry}
\geometry{verbose,a4paper,tmargin=1.75cm,bmargin=2cm,lmargin=2cm,rmargin=2cm,headheight=0.8cm,headsep=0.5cm,footskip=0.5cm}
\usepackage{babel}
\usepackage{url}
\urlstyle{same}
\newcommand\email{\begingroup \urlstyle{same}\Url}\usepackage{graphicx}
\usepackage{units}
\usepackage{tabularx}
\usepackage{wasysym} % enable use of \permil instead of \textperthousand
\usepackage{marvosym}
\usepackage{arabtex}
\usepackage{hyphenat}
\usepackage{booktabs}
\usepackage{mdwlist}
\renewcommand{\thefootnote}{\relax}
\usepackage{index}
\newindex{key}{kdx}{knd}{Keywords}
\newindex{author}{idx}{ind}{Authors}
\usepackage{fancyhdr}
\pagestyle{fancy}
\renewcommand{\headrulewidth}{0pt}
\fancyfoot{}
\usepackage{ifpdf}
\ifpdf
%\usepackage[activate]{pdfcprot}
%\usepackage[owner=Tapioka, print=true, copy=true, edit=false, annotate=false,set]{pdfcrypt}
%encryption: not supported any more (since pdftex 1.10)
\usepackage[pdftex]{thumbpdf}
\usepackage[pdftex]{hyperref}
\hypersetup{pdftitle={The Genetic Polymorphism of Tgf-$\beta$2 Gene in Indonesian Native Chickens},pdfauthor={Muhammad Muhsinin, Cece Sumantri, Asep Gunawan},pdfsubject={Tropentag 2016: Abstract},pdfkeywords={Genetic polymorphism, native chickens, PCR-RFLP, TGF-&#946;2 gene},pdfpagemode=None,colorlinks=true}
\else
\usepackage{ae}
\usepackage{textcomp}
\fi
\begin{document}
\ifpdf
\parbox[b]{2.5cm}{\resizebox*{2.5cm}{!}{\includegraphics{dtt2016.jpg}}}
\else
\parbox[b]{2.5cm}{\resizebox*{2.5cm}{!}{\includegraphics{dtt2016.eps}}}
\fi
\hfill
\parbox[b]{13.4cm}{\centering \large{\textbf{Tropentag, September 18-21, 2016, Vienna, Austria}}\\[1ex] \Large{``Solidarity in a competing world ---\\fair use of resources''\\[2ex]}}
\hspace{0.1cm}
\begin{center}
\Large{\textbf{The Genetic Polymorphism of Tgf-$\beta$2 Gene in Indonesian Native Chickens\footnote{\textbf{Contact Address:} Cece Sumantri, Bogor Agricultural University, Animal Production and Technology, Jl. Agatis Kampus IPB Darmaga, 16680~Bogor, Indonesia, \mbox{e-mail}: \email{cece_sumantri12@yahoo.co.id}}\\[0.8ex]}}
\normalsize{\textsc{Muhammad Muhsinin$^{1}$, Cece Sumantri$^{2}$, Asep Gunawan$^{3}$}}
\end{center}
\begin{itemize*}
\item[]{\small{\textit{$^{1}$Bogor Agricultural University, Animal Production and Technology, }}}
\item[]{\small{\textit{$^{2}$Bogor Agricultural University, Animal Production and Technology, Indonesia}}}
\item[]{\small{\textit{$^{3}$Bogor Agriculture University, Animal Production and Technology, Indonesia}}}
\end{itemize*}
\index[author]{Muhsinin, Muhammad}
\index[author]{Sumantri, Cece}
\index[author]{Gunawan, Asep}
\begin{center}
\vspace{1.1cm}
\textbf{Abstract}
\begin{abstract}
\normalsize{
Indonesia is a country rich in genetic resources, but its potential is still not optimally assessed. One of the potential of livestock genetic resources is the native chickens that have a high enough genetic variation. The transforming growth factor $\beta$ (TGF-$\beta$) superfamily consists of multifunctional cytokines that promote cell growth and extracellular matrix synthesis and mediate immune responses. TGF-$\beta$2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. The chicken TGF-$\beta$2 gene is located on chromosome 3 and is approximately 70 kb long with seven exons. A novel single nucleotide polymorphism (SNP) has been identified in the promoter region of TGF-$\beta$2 that had a positive effect on chicken growth. A few studies have also reported an association between this chicken TGF-$\beta$2 polymorphism and resistance to S. Enteritidis. The aim of this study was to identify the genetic variability of TGF-$\beta$2 gene in Indonesian native chicken using polymerase chain reaction"=restriction fragment polymorphism (PCR-RFLP) method. A total of 215 native chicken were identified RsaI (TGF-$\beta$2) restriction enzymes. TGF-$\beta$2 gene exon 1 amplifications resulted fragments with the lengths of 284 bp. Genotyping of TGF-$\beta$2 gene exon 1 produced three genotypes (CC, CT and TT). For the TGF-$\beta$2 gene, frequencies of T allele were dominant to the C allele across all populations. The results showed that TGF-$\beta$2|RsaI gene were polymorphics. Heterozygosity values were found low, except the TGF-$\beta$2 exon 1 in sentul chicken (0.50). The observed polymorphism in this study shows the ability of this candidate gene in marker assisted selection.}

\vspace{0.5cm}
\end{abstract}
\end{center}
\noindent \textbf{Keywords:} Genetic polymorphism, native chickens, PCR-RFLP, TGF-$\beta$2 gene
\index[key]{Genetic polymorphism}
\index[key]{Native chickens}
\index[key]{PCR-RFLP}
\index[key]{TGF-$\beta$2 gene}
\end{document}
