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\hypersetup{pdftitle={Ghrelin Receptor (GHSR) Gene Polymorphism in Indonesian Local Chicken and Crossbred Associated with Carcass Composition},pdfauthor={Isyana Khaerunnisa, Jakaria Jakaria, Irma Isnafia Arief, Cahyo Budiman, Cece Sumantri},pdfsubject={Tropentag 2016: Abstract},pdfkeywords={Carcass composition, crossbred, GHSR gene, Indonesian local chicken},pdfpagemode=None,colorlinks=true}
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\parbox[b]{13.4cm}{\centering \large{\textbf{Tropentag, September 18-21, 2016, Vienna, Austria}}\\[1ex] \Large{``Solidarity in a competing world ---\\fair use of resources''\\[2ex]}}
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\Large{\textbf{Ghrelin Receptor (GHSR) Gene Polymorphism in Indonesian Local Chicken and Crossbred Associated with Carcass Composition\footnote{\textbf{Contact Address:} Isyana Khaerunnisa, Bogor Agricultural University, Graduate School, Animal Production and Technology, Jl. Agatis Kampus Ipb Darmaga, 16680~Bogor, Indonesia, \mbox{e-mail}: \email{isyanakhaerunnisa@gmail.com}}\\[0.8ex]}}
\normalsize{\textsc{Isyana Khaerunnisa, Jakaria Jakaria, Irma Isnafia Arief, Cahyo Budiman, Cece Sumantri}}
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\begin{itemize*}
\item[]{\small{\textit{Bogor Agricultural University, Graduate School, Animal Production and Technology, Indonesia}}}
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\index[author]{Khaerunnisa, Isyana}
\index[author]{Jakaria, Jakaria}
\index[author]{Arief, Irma Isnafia}
\index[author]{Budiman, Cahyo}
\index[author]{Sumantri, Cece}
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\textbf{Abstract}
\begin{abstract}
\normalsize{
Ghrelin receptor (GHSR) gene is candidate gene for growth performance in chicken. This gene modulates growth hormone release from the pituitary by binding to its ligand of ghrelin. Ghrelin gene, or growth hormone secretagogue (GHS) gene, is well known as feed intake and energy homeostasis regulator in mammals and birds. Indonesia has a wide variety of local chickens that play important role in national food security and self"=sufficiency. The objectives of this study were to identify the polymorphism of intron 1 GHSR gene in Indonesian local chicken and to evaluate its effects on carcass composition. The gene polymorphism was identified with polymerase chain reaction"=restriction fragment length polymorphism (PCR-RFLP) method by using Hin6I restriction enzyme. Effect of genotype on carcass composition was analysed using SAS General Linear Model (GLM) procedure. The genotyping was performed on 343 individuals including 7 Indonesian local chicken and crossbred populations (\textit{Merawang, Pelung, Sentul, Kampung, Cobb} broiler, F1 crossbred of Kampung\,$\times$\,Cobb broiler, and F2 crossbred of Kampung\,$\times$\,Cobb broiler). All individuals were successfully amplified in GHSR|Hin6I locus, resulting in a fragment with 470 bp PCR product. This locus was polymorphic with two alleles (T and C) and derived three genotypes (TT, CT and CC). The T allele was major allele in \textit{Merawang, Pelung, Sentul, Kampung, Cobb} broiler, F1 crossbred of \textit{Kampung} x \textit{Cobb} broiler, F2 crossbred of \textit{Kampung} x \textit{Cobb }broiler and overall population (0.781, 0.717, 0.944, 0.949, 0.838, 0.869, 0.884 and 0.864, respectively). The TT genotype frequency was found at the highest in \textit{Merawang, Pelung, Sentul, Kampung, Cobb }broiler and overall population (0.596, 0.478, 0.889, 0.918, 0.676, 0.738, 0.768 and 0.741, respectively). Individuals with CT genotype had significantly higher live weight at 26 weeks, carcass weight, commercial cuts weights, and muscles weights than TT genotype in F2 crossbred of \textit{Kampung}\,$\times$\,\textit{Cobb} broiler population. Association of GHSR|Hin6I gene polymorphism with chicken carcass composition have been described in Indonesian chicken, providing evidence that GHSR might be an important candidate gene for chicken carcass composition.}

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\noindent \textbf{Keywords:} Carcass composition, GHSR gene, Indonesian local chicken
\index[key]{Carcass composition}
\index[key]{Crossbred}
\index[key]{GHSR gene}
\index[key]{Indonesian local chicken}
\index[key]{Chicken}
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