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\hypersetup{pdftitle={Hydrogen Peroxide and Salicylic Acid Are Involved in Podophyllotoxin  Production in Linum Album Cell Cultures},pdfauthor={Safieh Fakhari, Mohsen Sharifi, Faezeh Ghanati},pdfsubject={Tropentag 2016: Abstract},pdfkeywords={ Salicylic Acid, Imidazole, Linum album, podophyllotoxin},pdfpagemode=None,colorlinks=true}
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\parbox[b]{13.4cm}{\centering \large{\textbf{Tropentag, September 18-21, 2016, Vienna, Austria}}\\[1ex] \Large{``Solidarity in a competing world ---\\fair use of resources''\\[2ex]}}
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\Large{\textbf{Hydrogen Peroxide and Salicylic Acid Are Involved in Podophyllotoxin  Production in Linum Album Cell Cultures\footnote{\textbf{Contact Address:} Safieh Fakhari, Tarbiat Modares University, Department of Plant Biology, Gysha Bridge, Tehran, Iran, \mbox{e-mail}: \email{safiehfakhari@gmail.com}}\\[0.8ex]}}
\normalsize{\textsc{Safieh Fakhari$^{1}$, Mohsen Sharifi$^{2}$, Faezeh Ghanati$^{3}$}}
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\begin{itemize*}
\item[]{\small{\textit{$^{1}$Tarbiat Modares University, Department of Plant Biology, Iran}}}
\item[]{\small{\textit{$^{2}$Tarbiat Modares University, Department of Plant Biology, }}}
\item[]{\small{\textit{$^{3}$Tarbiat Modares University, Department of Plant Biology, }}}
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\index[author]{Fakhari, Safieh}
\index[author]{Sharifi, Mohsen}
\index[author]{Ghanati, Faezeh}
\begin{center}
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\textbf{Abstract}
\begin{abstract}
\normalsize{
Cell suspension cultures of Linum album Kotschy ex Boiss. have been reported to produce anticancer podophyllotoxin and its related lignans. Hydrogen peroxide and salicylic acid are key signaling molecules in cells whose levels are increased in response to various stimuli and are involved in plant secondary metabolite synthesis. In order to investigate the relationship and/or interactions of salicylic acid and reactive oxygen species in PTOX biosynthesis of Linum album suspension cells, we treated the cells with Imidazole (IMD), an inhibitor of NADPH oxidase in the plasma membrane, which can inhibit the production of H2o$_{2}$ by inactivating NPDPH oxidase. In single treatment experiments, sterile IMD in concentrations (0, 50, 100, 250 $\mu$M) were added to 7-day"=old cell cultures and were harvested after 48 hours. In the best concentration of IMD (50 $\mu$M), signaling molecules include H2o$_{2}$ and SA in time course (0,1,3,6 and 9 h) were investigated. The results showed that minimum PTOX production was achieved by the addition of 50 $\mu$M IMD, 2.9 $\mu$g/g FW compared to control 7.2 $\mu$g/g FW. Analyses showed that in IMD-treated cells, the phenylalanine ammonia"=lyase (PAL) and cinnamyl"=alcohol dehydrogenase (CAD) activity, which involved in the first steps of PTOX biosynthesis, also decreased after the treatment. Treatment of the cells with Imidazole decreased not only hydrogen peroxide generation and SA accumulation but also the PTOX production, which suggests that both hydrogen peroxide and SA are involved in PTOX biosynthesis. The action of IMD on these metabolites and signaling molecules can be applied to control the biotechnological production of this anticancer agent.}

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\noindent \textbf{Keywords:}  Salicylic Acid, Imidazole, Linum album, podophyllotoxin
\index[key]{ Salicylic Acid}
\index[key]{Imidazole}
\index[key]{Linum album}
\index[key]{Podophyllotoxin}
\end{document}
