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\hypersetup{pdftitle={Genome Wide Association for two Glucosinolates Compounds in Cauliflower},pdfauthor={Eltohamy Ali Ahmed Yousef},pdfsubject={Tropentag 2016: Abstract},pdfkeywords={Cauliflower, Genome wide association, Glucosiolates },pdfpagemode=None,colorlinks=true}
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\parbox[b]{13.4cm}{\centering \large{\textbf{Tropentag, September 18-21, 2016, Vienna, Austria}}\\[1ex] \Large{``Solidarity in a competing world ---\\fair use of resources''\\[2ex]}}
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\Large{\textbf{Genome Wide Association for two Glucosinolates Compounds in Cauliflower\footnote{\textbf{Contact Address:} Eltohamy Ali Ahmed Yousef, Suez Canal University, Faculty of Agriculture, Horticulture, 41522~Ismailia, Egypt, \mbox{e-mail}: \email{tohamyy@yahoo.com}}\\[0.8ex]}}
\normalsize{\textsc{Eltohamy Ali Ahmed Yousef}}
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\item[]{\small{\textit{Suez Canal University, Faculty of Agriculture, Horticulture, Egypt}}}
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\index[author]{Yousef, Eltohamy Ali Ahmed}
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\textbf{Abstract}
\begin{abstract}
\normalsize{
Cauliflower (Brassica oleracea var. botrytis) enjoys a high reputation among proponents of healthy nutrition and sustainable food production. One reason for the popularity of cauliflower is the high glucosinolate content, which has anti"=cancerogenic properties. Genome wide association (GWAS) allows exploiting natural variation to reveal genetic control of complex traits. The goal of this study was to explore the potential of GWAS for improving glucosinolate content trait in cauliflower. To achieve this goal, fifty accessions of cauliflower were ordered from genebanks of the United states Department of Agriculture (USDA), USA and the Leibniz-Istitut fur Pflanzengenetik und Kulturpflanzenforschung (IPK) Gatersleben, Germany. The used materials were genotyped with genotyping"=by-sequencing (GBS).  Field trial was carried out at two locations in Stuttgart, Germany: Heidfeldhof (conventional farm) and Kleinhohenheim (organic farm). One plant per accession was used to extract glucosinolates compounds from each location. Two samples per plant were prepared for measuring by High"=performance liquid chromatography (HPLC). Each sample was measured three times by HPLC. Two compounds were measured: singrin and glucobrassicin (ng/ml). EMMAX with 43182 SNPs was used for GWAS on two traits. Cauliflower accessions show high diversity for singrin and glucobrasscin content and genotype was the main source of total variations for both traits, indicating to the ability to improve these compounds through the breeding program. SNPs associated with singrin and glucobrassicin content were detected using GWAS. The results suggest that GWAS can be applied to genetically diverse genebank materials to identify useful quantitative trait loci (QTL) and genotypes for utilisation as genetic resources in cauliflower breeding.







}

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\noindent \textbf{Keywords:} Cauliflower, Genome wide association, Glucosiolates 
\index[key]{Cauliflower}
\index[key]{Genome wide association}
\index[key]{Glucosiolates }
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