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\hypersetup{pdftitle={Nutritive Value and Biomass Production of \textit{Brachiaria humidicola} Hybrids with Divergent Biological Nitrification Inhibition Activity},pdfauthor={Mario Cuchillo Hilario, Danilo Moreta, Jacobo Arango, Johanna Mazabel, Paola Pardo, Stiven Quintero, Mauricio Efren Sotelo Cabrera, Margaret Worthington, Idupulapati Rao, John  Miles, Michael Peters},pdfsubject={Tropentag 2016: Abstract},pdfkeywords={BNI, Brachiaria humidicola, nitrous oxide, tropical grasses},pdfpagemode=None,colorlinks=true}
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\begin{document}
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\parbox[b]{13.4cm}{\centering \large{\textbf{Tropentag, September 18-21, 2016, Vienna, Austria}}\\[1ex] \Large{``Solidarity in a competing world ---\\fair use of resources''\\[2ex]}}
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\Large{\textbf{Nutritive Value and Biomass Production of \textit{Brachiaria humidicola} Hybrids with Divergent Biological Nitrification Inhibition Activity\footnote{\textbf{Contact Address:} Mario Cuchillo Hilario, National Institute of Medical Sciences and Nutrition 'Salvador Zubirán' (INCMNSZ), Animal Nutrition Department, Mexico City, Mexico, \mbox{e-mail}: \email{mario.cuchilloh@incmnsz.mx}}\\[0.8ex]}}
\normalsize{\textsc{Mario Cuchillo Hilario$^{1,2}$, Danilo Moreta$^{2}$, Jacobo Arango$^{2}$, Johanna Mazabel$^{2}$, Paola Pardo$^{2}$, Stiven Quintero$^{2}$, Mauricio Efren Sotelo Cabrera$^{2}$, Margaret Worthington$^{2}$, Idupulapati Rao$^{2}$, John  Miles$^{2}$, Michael Peters$^{2}$}}
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\begin{itemize*}
\item[]{\small{\textit{$^{1}$National Institute of Medical Sciences and Nutrition 'Salvador Zubirán' (INCMNSZ), Animal Nutrition Department, Mexico}}}
\item[]{\small{\textit{$^{2}$International Center for Tropical Agriculture (CIAT), Colombia}}}
\end{itemize*}
\index[author]{Cuchillo Hilario, Mario}
\index[author]{Moreta, Danilo}
\index[author]{Arango, Jacobo}
\index[author]{Mazabel, Johanna}
\index[author]{Pardo, Paola}
\index[author]{Quintero, Stiven}
\index[author]{Sotelo Cabrera, Mauricio Efren}
\index[author]{Worthington, Margaret}
\index[author]{Rao, Idupulapati}
\index[author]{Miles, John }
\index[author]{Peters, Michael}
\begin{center}
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\textbf{Abstract}
\begin{abstract}
\small{
Tropical land areas utilised for feed production are under continuous pressure to provide sufficient feed in terms of biomass and nutrient quality. Also, some \textit{Brachiaria humidicola} (Bh) genotypes have been identified with high Biological Nitrification Inhibition (BNI) activity. The BNI trait may reduce the rate of transformation of ammonia into nitrate in soils and could contribute to mitigation of climate change by avoiding the formation of nitrate and later moderating nitrous oxide emission during denitrification. Twelve intraspecific hybrids of Bh with contrasting BNI activity were classified into 1) high (0.58--0.96\,mg NO$_{3}^{}$\,kg\textsuperscript{-1}soil\,day\textsuperscript{-1}), 2) intermediate (1.27--1.62\,mg NO$_{3}^{}$\,kg\textsuperscript{-1}soil\,day\textsuperscript{-1})) and low (1.84--2.49\,mg NO$_{3}^{}$\,kg\textsuperscript{-1}soil\,day\textsuperscript{-1})) activity. Five Bh genotypes from CIAT's forage germplasm collection (CIAT 679, CIAT 26146, CIAT 26149, CIAT 26159, CIAT 16888) were employed as controls. Seventeen plots (4\,$\times$\,4m$^{2}$) with three replications were use to allocate the experimental units. Each single plot was divided into eight subplots (0.5\,$\times$\,0.5\,m$^{2}$). Rising"=plate-meter (RPM, \mbox{30\,cm} diameter) was used to measure successively the compressed sward height (CSH) of pasture on each subplot at 0, 7, 14, 21, and 28 days after pasture height homogenisation. Eight RPM-heights were taken on each subplot and were averaged. Biomass was collected at two points below the disc (one sample under and one sample above CSH average) of the RPM to ground level after CSH measurement. Biomass was dried for 48 hours at 60°C and ground (1mm) for further analysis. Near infrared spectroscopy (Foss 6500) was used to calculate crude protein (CP), neutral detergent fiber (NDF), NDF digestibility (NDFd), acid detergent fiber (ADF), and in"=vitro dry matter digestibility (IVDMD). Ten percent of the samples were randomly assessed by wet chemistry for NIRS validation. The randomised complete block design was employed for data analysis (SPSS-v20). Bh hybrids with intermediate BNI activity had the largest biomass production (3208\,kg\,DM\,ha\textsuperscript{-1}) and which differed from that of the hybrids with either high of low BNI activity (2916 or 2800\,kg\,DM\,ha\textsuperscript{-1}, respectively). In contrast, no differences among the BNI groupings were observed for CP, NDF, ADF, NDFd, or IVDMD. Further evaluations across seasons, years, and locations, are recommended to confirm and extend these findings.}

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\noindent \textbf{Keywords:} BNI, \textit{Brachiaria humidicola}, nitrous oxide, tropical grasses
\index[key]{BNI}
\index[key]{Brachiaria humidicola@\textit{Brachiaria humidicola}}
\index[key]{Nitrous oxide}
\index[key]{Tropical grasses}
\end{document}
