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\hypersetup{pdftitle={Application of Microsatellite Markers for Parentage Determination of the Shrimp Penaeus Semisulcatus},pdfauthor={Mohamed Megahed},pdfsubject={Tropentag 2016: Abstract},pdfkeywords={DNA parentage, Fenneropeanus indicus, Microsatellites, Paternity, Penaeus semisulcatus, shrimp},pdfpagemode=None,colorlinks=true}
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\parbox[b]{13.4cm}{\centering \large{\textbf{Tropentag, September 18-21, 2016, Vienna, Austria}}\\[1ex] \Large{``Solidarity in a competing world ---\\fair use of resources''\\[2ex]}}
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\Large{\textbf{Application of Microsatellite Markers for Parentage Determination of the Shrimp Penaeus Semisulcatus\footnote{\textbf{Contact Address:} Mohamed Megahed, National Institute of Oceanography and Fisheries, Aquaculture, Gulfs of Suez & Aqaba's Branch,, 43511~Attaka, Suez, , Egypt, \mbox{e-mail}: \email{aquageimprove@gmail.com}}\\[0.8ex]}}
\normalsize{\textsc{Mohamed Megahed}}
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\begin{itemize*}
\item[]{\small{\textit{National Institute of Oceanography and Fisheries, Aquaculture, Egypt}}}
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\index[author]{Megahed, Mohamed}
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\textbf{Abstract}
\begin{abstract}
\normalsize{
The recognition that reliance on wild broodstock for shrimp production in Egypt is non"=sustainable. Nowadays great efforts are encouraged to domesticate various shrimp species available in coastal water of Egypt in order to improve the sustainability of post larval supplies, reduce risks of disease and gain benefits from selective breeding. Along with domestication comes the opportunity to increase the productivity of shrimp farming through genetic improvement. A first commercial breeding programme for Penaeus smeisulcatus and Fenneropenaues indicus started in 2010 to improve the shrimp production in Egypt.This work aims at developing microsatellite markers for the use in commercial selective breeding of the shrimp P.semisulcatus in Egypt. In this study we used controlled matings and computer programs to examine the potential of microsatellite markers in assigning parentage to P.semisulcatus progeny. Fifty three families were produced and reared separately in larval rearing tanks. The data collected in hand is Dam ID, offspring tank ID, but male information is missing. This missing information prevents us to construct pedigree of the different families. 100 microsatellites were screened in the produced families. 10 microsatellites was successful to assign progeny to their correct parents (with \mbox{98\,\%}). This application of microsatellites in the breeding of P.semisulcatus proofed as a tool to retain pedigree information in shrimp selective breeding programs. One of the major benefits of applying DNA parentage analyses to shrimp breeding programs is that families can be communally reared from birth minimising potential confounding genetic and environmental influences. BDNA parentage analysis has been routinely applied to breeding programs in P.semisulcatus. Future research needs to fins additional markers to be developed and evaluated to increase the success of allocation. Also, we need to try application of these markers in other species of farmed shrimp.











}

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\noindent \textbf{Keywords:} DNA parentage, Fenneropeanus indicus, Microsatellites, Paternity, Penaeus semisulcatus, shrimp
\index[key]{DNA parentage}
\index[key]{Fenneropeanus indicus}
\index[key]{Microsatellites}
\index[key]{Paternity}
\index[key]{Penaeus semisulcatus}
\index[key]{Shrimp}
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