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\hypersetup{pdftitle={Genome Wide Association Studies for Exploring Salt Tolerance in Barley},pdfauthor={Oscar Nnaemeka Obidiegwu, Rajiv Sharma, Benjamin Kilian, Willmar L. Leiser, Folkard Asch},pdfsubject={Tropentag 2016: Abstract},pdfkeywords={Association mapping, Hordeum vulgare, QTL, salt tolerance, SNP},pdfpagemode=None,colorlinks=true}
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\parbox[b]{13.4cm}{\centering \large{\textbf{Tropentag, September 18-21, 2016, Vienna, Austria}}\\[1ex] \Large{``Solidarity in a competing world ---\\fair use of resources''\\[2ex]}}
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\Large{\textbf{Genome Wide Association Studies for Exploring Salt Tolerance in Barley\footnote{\textbf{Contact Address:} Oscar Nnaemeka Obidiegwu, University of Hohenheim, Inst. of Agricultural Sciences in the Tropics (Hans-Ruthenberg-Institute), Garbenstr. 13, 70599~Stuttgart, Germany, \mbox{e-mail}: \email{oscaro@uni-hohenheim.de}}\\[0.8ex]}}
\normalsize{\textsc{Oscar Nnaemeka Obidiegwu$^{1}$, Rajiv Sharma$^{2}$, Benjamin Kilian$^{2}$, Willmar L. Leiser$^{3}$, Folkard Asch$^{1}$}}
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\begin{itemize*}
\item[]{\small{\textit{$^{1}$University of Hohenheim, Inst. of Agricultural Sciences in the Tropics (Hans-Ruthenberg-Institute), Germany}}}
\item[]{\small{\textit{$^{2}$Leibniz-institute of Plant Genetics and Crop Plant Research (IPK), Genome Diversity, Germany}}}
\item[]{\small{\textit{$^{3}$University of Hohenheim, Inst. of Plant Breeding, Seed Sciences and Population Genetics, Germany}}}
\end{itemize*}
\index[author]{Obidiegwu, Oscar Nnaemeka}
\index[author]{Sharma, Rajiv}
\index[author]{Kilian, Benjamin}
\index[author]{Leiser, Willmar L.}
\index[author]{Asch, Folkard}
\begin{center}
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\textbf{Abstract}
\begin{abstract}
\small{
Salt stress poses an increasing threat to barley production. Salt is taken up passively and distributed within the crop via its transpiration stream. Thus uptake of salt is directly related to stomatal responses to vapour pressure deficit (VPD) perceived as atmospheric drought signal. Due to the variability in the timing and severity of salt stress in addition to less reliable seasonal patterns associated with climate change, salinity effects on the crop may increase at several development stages of the crop. Salt tolerance is a complex trait characterised by interactions between genes and their environment. Consequently genotypes grown in varying VPD environment tend to respond differently to salt stress. Hence identifying patterns of polymorphism in the genome that are suggestive of the effect of the risk enhancing or protective alleles has been a major challenge in the quest to develop salt tolerant crop varieties. This research aims at understanding the genetic basis for genotypic adaptation in barley to combinations of salt and VPD stress at different crop development stages. A diverse set of 216 spring barley accessions of worldwide origin (constituting of six row and two row spike morphology types) were screened at emergence and early seedling stage at 250 mM NaCl concentrations and control. Subsequently, these genotypes were grown on hydroponics within a phenotyping platform and were exposed to varying VPD levels (0.73 and 1.85 kpa) in addition to salt level of 200 mM NaCl. Using genome wide association studies (GWAS) employing 9K SNP markers (iSelect assay), we explore the genetic variation for salt tolerance in barley for traits such as seed germination rate, mobilisation efficiency of endosperm reserves, biomass accumulation and partitioning, tiller number, leaf area and leaf ion balance (Na$^{+}$, K+) for the aforementioned stress combination. We observed broad genetic variation for salt stress tolerance. Relative responses due to salt and VPD stresses will be presented and the potentials for efficient screening for salt tolerance will be discussed. Findings on QTLs would be validated with previous research and reported.}

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\noindent \textbf{Keywords:} Association mapping, \textit{Hordeum vulgare}, QTL, salt tolerance, SNP
\index[key]{Association mapping}
\index[key]{Hordeum vulgare@\textit{Hordeum vulgare}}
\index[key]{QTL}
\index[key]{Salt!tolerance}
\index[key]{SNP}
\end{document}
