Rhizogenezis of Leafy Stem Cuttings of Calycophyllum spruceanum (Bentham) Hooker F. Ex Schumann: Effect of Indol-3-Butyric Acid
Jiri Lipensky, Bohdan Lojka
Czech University of Life Sciences Prague, Dept. of Crop Sciences and Agroforestry in the Tropics and Subtropics, Czech Republic
Reforestation and domestication of forest trees in the Peruvian Amazon depends especially on succesful propagation of partical species. Vegetative propagation may serve as a tool for selected genotype conservation, and is an alternative to propagation by seeds, which is often constrained. This study focused on determining effects of five different concentrations of Indole-3-butyric acid (IBA) on rooting parametres of leafy stem semihard wood cuttings of Calycophyllum spruceanum (Rubiaceae). This fast growing pioneer tree species is valued by local people not only for its relatively good quality wood with straight growth habit, but also for its medicinal purposes. After 21 days in sub-irrigated polyethylene polypropagator, rooting parametres of the propagules were measured. The number and percentage of rooting, callus formation, number of roots, total root length, and length of the longest root was significantly higher, while mortality and leaf abscission was significantly lower in group treated with 2000 ppm of IBA than in control group with untreated cuttings (P < 0.05). However no significant differences were found in these parametres between the group treated with 2000 ppm and groups treated with 4000 and 5000 ppm, respectively, except for the leaf abscission parametre. No significant differences were recorded in number of callusses per cutting between the five groups tested. These results shows that rhizogenesis of C. spruceanum was influenced by the application of IBA. The best concentration for successive vegetative propagation of juvenile leafy stem semihardwood cuttings of this species was 2000 ppm of IBA.
Keywords: Domestication of timber species, Indole-3-butyric acid (IBA), leafy stem cutting, subirigated polyethylene polypropagator
Contact Address: Jiri Lipensky, Czech University of Life Sciences Prague, Department of Crop Sciences and Agroforestry in Tropics and Subtropics, Kamycka, 165 21 Prague, Czech Republic, e-mail: lipenskyits.czu.cz