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\hypersetup{pdftitle={Determination of the Phytase Activity and Phytate P of Quinoa (\textit{Chenopodium quinoa} Willd.) using Spectrophotometric  and Isotachophoretic Methods},pdfauthor={Olga Lucia Rosero Alpala, Milan Marounek, Natalie Brenová, Daniela Lukesova},pdfsubject={Tropentag 2010: Abstract},pdfkeywords={Antinutritionals factors, endogenous enzyme activity, phytic acid, quinoa},pdfpagemode=None,colorlinks=true}
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\parbox[b]{13.4cm}{\centering \large{\textbf{Tropentag, September 14-16, 2010, Zurich}}\\[1ex] \Large{``World Food System  ---\\A Contribution from Europe''\\[2ex]}}
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\Large{\textbf{Determination of the Phytase Activity and Phytate P of Quinoa (\textit{Chenopodium quinoa} Willd.) using Spectrophotometric  and Isotachophoretic Methods\footnote{\textbf{Contact Address:} Olga Lucia Rosero Alpala, Czech University of Life Sciences Prague, Institute of Tropics and Subtropics, Kamycka 1072 Praha 6, 16521~Prague, Czech Republic, \mbox{e-mail}: \email{olroseroa@unal.edu.co}}\\[0.8ex]}}
\normalsize{\textsc{Olga Lucia Rosero Alpala$^{1}$, Milan Marounek$^{2}$, Natalie B\v{r}e\v{n}ová$^{2}$, Daniela Lukesova$^{1}$}}
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\begin{itemize*}
\item[]{\small{\textit{$^{1}$Czech University of Life Sciences Prague, Institute of Tropics and Subtropics, Czech Republic}}}
\item[]{\small{\textit{$^{2}$Czech Academy of Sciences, Institute of Animal Physiology and Genetics, Czech Republic}}}
\end{itemize*}
\index[author]{Rosero Alpala, Olga Lucia}
\index[author]{Marounek, Milan}
\index[author]{B\v{r}e\v{n}ová, Natalie}
\index[author]{Lukesova, Daniela}
\begin{center}
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\textbf{Abstract}
\begin{abstract}
\normalsize{
The quinoa (\textit{Chenopodium quinoa} Willd.), is a pseudocereal that has been cultivated in the Andean region in Nariño, Colombia and have great opportunity in the improving food and feed mainly on its use as feedstuff. The aims of this work was to analyse the P contents, phytate phosphorus contents and phytase activity of Quinoa Nariño (QC); Quinoa Anapqui's (QBA); Quinoa -IICA 020 Oruro (QB); Quinoa Huancavelica (QP) and wheat (control) were also analysed at physiological temperature (39\textdegree{}C) and 37\textdegree{}C adopted for additives regulations. Phytic acid was determined by capillary isotachophoretic method and the phytase activity was estimated by a spectrophotometric method. Phosphorus in this study found in major level in QP"=Huancavelica variety (508 mg g\textsuperscript{-1}). The phytic acid P proportion in the total P was significantly ($P<0.05$) low in QC (19.64\,\%), QBA (26.95\,\%), QB (33.6\,\%) and QP (32.17\,\%) varieties analysed in comparison with wheat (60.22\,\%) evaluated.



The phytic acid P proportion in the total P was low in QC (19,\mbox{64\,\%}), QBA (\mbox{26.95\,\%}), QB (\mbox{33.6\,\%}) and QP (\mbox{32.17\,\%}) varieties analysed in comparison with the other cereals evaluated. The phytase activity was evaluated at physiological (39\textdegree{}C) temperature and at standard (37\textdegree{}C), the phytase activity was effected by the temperature in QC (1153\,{FTU}\,{kg}\textsuperscript{-1}, 1152\,{FTU}\,{kg}\textsuperscript{-1}), QBA (847 and 593\,{FTU}\,{kg}\textsuperscript{-1}), QB (896 and 613\,{FTU}\,{kg}\textsuperscript{-1}), QP (909 and 561\,{FTU}\,{kg}\textsuperscript{-1}) and wheat control (1137 and 1046\,{FTU}\,{kg}\textsuperscript{-1}) under 39 and 37\textdegree{}C, respectivily. The Bolivian varieties did not present any significant differences. Presented negative correlation (-0.89 at 37\textdegree{}C and -0.79 at 39\textdegree{}C) was established between the phytase activity and the amount of phytic acid P among the four samples. To conclude, the results showed a great potential of the quinoa grains due to the low level of phytic acid P and high phytase activity.}

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\noindent \textbf{Keywords:} Antinutritionals factors, endogenous enzyme activity, phytic acid, quinoa
\index[key]{Antinutritionals factors}
\index[key]{Phytic acid}
\index[key]{Quinoa}
\index[key]{Endogenous enzyme activity}
\end{document}