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\hypersetup{pdftitle={The Role of the Sulfur Nutrition on Genotypic Differences in the Resistance of Tomato against \textit{Botrytis cinerea}},pdfauthor={Habtamu Bekele Demissie, Walter Horst, Marc Zahn},pdfsubject={Tropentag 2010: Abstract},pdfkeywords={Pathogen resistance, SED, SDCs, Sulfur nutrition},pdfpagemode=None,colorlinks=true}
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\parbox[b]{13.4cm}{\centering \large{\textbf{Tropentag, September 14-16, 2010, Zurich}}\\[1ex] \Large{``World Food System  ---\\A Contribution from Europe''\\[2ex]}}
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\Large{\textbf{The Role of the Sulfur Nutrition on Genotypic Differences in the Resistance of Tomato against \textit{Botrytis cinerea}\footnote{\textbf{Contact Address:} Habtamu Bekele Demissie, Leibniz Universität Hannover, Institute of Plant Nutrition, Heidjerhof 3 Zi 202, 30625~Hannover, Germany, \mbox{e-mail}: \email{habitish05@yahoo.com}}\\[0.8ex]}}
\normalsize{\textsc{Habtamu Bekele Demissie, Walter Horst, Marc Zahn}}
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\begin{itemize*}
\item[]{\small{\textit{Leibniz Universität Hannover, Institute of Plant Nutrition, Germany}}}
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\index[author]{Demissie, Habtamu Bekele}
\index[author]{Horst, Walter}
\index[author]{Zahn, Marc}
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\textbf{Abstract}
\begin{abstract}
\normalsize{
Tomato (\textit{Solanum lycopersicum}) is the second most consumed vegetable worldwide. However, the production of tomato is hampered by different biotic and abiotic stresses of which \textit{Botrytis cinerea }is one of the biotic factors. Tomato is affected by B. cinerea due to lack of resistance genes in the cultivated tomato plant. The resistance by tomato plant against B. cinerea can be improved by an enhanced formation of S-containing defence compounds (SDCs). General Objective:To contribute to the development of a tomato production system based on sulfur"=enhanced endogenous tolerance and defence mechanisms (SED) against the main fungal pathogen B. cinerea. Specific objectives: To increase the formation of SDCs by increasing the S supply and thus enhancing sulfate uptake leading to higher concentrations of S in the plant tissue that can be used in defence against B. cinerea (SED) and Exploiting genotypic differences in resistance against \textit{B. cinerea} in a \textit{S. lycopersicum} / \textit{S. habrochoides} IL population thus facilitating to elucidate the genetic basis of  resistance in tomato. Parents and 90 Introgression Lines (ILs) were grown in green house and provided with optimal and supra"=optimal sulphate S fertiliser and its resistance against B. cinerea has been analysed in"=vitro using droplet inoculation"=method. The formation of lesion has been determined with the GSA Image Analysis Software. The result shows additional S has significant effect in disease reduction. Few resistance lines have been selected following stastical analyisis (ANOVA, T-test). Total DNA of B. cinerea from the selected ILs and parents will be extracted with CTAB method. SYBR Green based real time PCR assays (absolute quantification, standard curve method) will be used to determine infection levels of the different ILs. SDCs (Glutathione, Cysteine, Thiosulphate, So, H$_{2}$S)from the selected ILs will also be analysed using HPLC, CNS analyser, and autoanalyzer. Major goal: is about the production of active sulfur containing defence compounds against Botrytis cinerea. Specific goal: is selection of resistance ILs, responsible in the resistance against this pathogen through the production of Sulphur defence compounds using \textit{in vitro }inoculation technique, GSA software, and molecular technique, qPCR.}

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\noindent \textbf{Keywords:} Pathogen resistance, SED, SDCs, Sulfur nutrition
\index[key]{Sulfur nutrition}
\index[key]{Pathogen!resistance}
\index[key]{Tomato}
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