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\hypersetup{pdftitle={Contribution to the Systematics of the Genus \textit{Centrosema} (Leguminosae) through Molecular Analyses: An Ongoing Project in Venezuela},pdfauthor={Solmeryhel Rodríguez, Gustavo Fermín, María Raymúndez, Antonieta Porco, Iselen Trujillo, Rainer Schultze-Kraft, Teodardo Calles},pdfsubject={Tropentag 2010: Abstract},pdfkeywords={Centrosema, introns, ITS, phylogenetics, RAPD, SSRs},pdfpagemode=None,colorlinks=true}
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\parbox[b]{13.4cm}{\centering \large{\textbf{Tropentag, September 14-16, 2010, Zurich}}\\[1ex] \Large{``World Food System  ---\\A Contribution from Europe''\\[2ex]}}
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\Large{\textbf{Contribution to the Systematics of the Genus \textit{Centrosema} (Leguminosae) through Molecular Analyses: An Ongoing Project in Venezuela\footnote{\textbf{Contact Address:} Teodardo Calles, Foundation Botanical Institute of Venezuela (FIBV), Caracas, Venezuela, \mbox{e-mail}: \email{teodardo.calles@uni-hohenheim.de}}\\[0.8ex]}}
\normalsize{\textsc{Solmeryhel Rodríguez$^{1}$, Gustavo Fermín$^{2}$, María Raymúndez$^{1}$, Antonieta Porco$^{3}$, Iselen Trujillo$^{4}$, Rainer Schultze-Kraft$^{5}$, Teodardo Calles$^{6}$}}
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\begin{itemize*}
\item[]{\small{\textit{$^{1}$Central University of Venezuela, Institute for Experimental Biology, Venezuela}}}
\item[]{\small{\textit{$^{2}$University of the Andes, Mérida Botanical Garden Center, Venezuela}}}
\item[]{\small{\textit{$^{3}$Simón Bolívar University, Department of Cell Biology, Venezuela}}}
\item[]{\small{\textit{$^{4}$Simón Rodríguez National University of Technology, Institute for Scientific and Technological Studies, Venezuela}}}
\item[]{\small{\textit{$^{5}$International Center for Tropical Agriculture (CIAT), Colombia}}}
\item[]{\small{\textit{$^{6}$Foundation Botanical Institute of Venezuela (FIBV), Venezuela}}}
\end{itemize*}
\index[author]{Rodríguez, Solmeryhel}
\index[author]{Fermín, Gustavo}
\index[author]{Raymúndez, María}
\index[author]{Porco, Antonieta}
\index[author]{Trujillo, Iselen}
\index[author]{Schultze-Kraft, Rainer}
\index[author]{Calles, Teodardo}
\begin{center}
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\textbf{Abstract}
\begin{abstract}
\normalsize{
\textit{Centrosema} is an economically important Neotropical genus which, together with \textit{Desmodium} and \textit{Stylosanthes}, is regarded as a genus with particularly high potential as a forage legume in the tropics and sub"=tropics. Several \textit{Centrosema} species are currently being used not only as forage but also for soil cover and soil improvement. Some species are well adapted to prolonged drought and the acid, low"=fertility soils that prevail in the tropics, thus making \textit{Centrosema} species particularly important for low"=input agricultural production systems.



There are some uncertainties regarding species delimitation and evolutionary relationships within the genus, due to the morphological plasticity of the species. Consequently, there is a number of differences between species which are not discernable using only morphological analysis. Therefore, the main objective of this research is to assess species delimitation and phylogenetic relationships among Venezuelan \textit{Centrosema} species, using molecular data, as a complement of morphological studies. Molecular analyses are based on polymorphism of intergenic sequences of cpDNA \textit{atp$\beta$-rbcL} and \textit{trnK/matK}, Internal Transcribed Spacer (ITS) of rDNA, Random Amplification of Polymorphic DNA (RAPD), and Simple Sequence Repeats (SSRs).



Preliminary results of the intergenic sequences \textit{trnK}/\textit{matK} (\textit{trnK}685F/\textit{matK}1932) and \textit{atp$\beta$-rbcL} (R7/S11) show, on the one hand, that \textit{Centrosema} has a monophyletic origin, \textit{i.e.}, the species have a common ancestor. On the other hand, the grouping of species (clades) obtained by these molecular markers differs, in some cases, from the classification proposed by Williams and Clements (1990) where the \textit{Centrosema} species were classified in 11 sub"=generic groups based on morphological similarities. More conclusive results are expected when further data are obtained.}

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\noindent \textbf{Keywords:} \textit{Centrosema}, introns, ITS, phylogenetics, RAPD, SSRs
\index[key]{Centrosema@\textit{Centrosema}}
\index[key]{Introns}
\index[key]{ITS}
\index[key]{Phylogenetics}
\index[key]{RAPD}
\index[key]{SSRs}
\end{document}