Tropentag, October 6 - 8, 2009 in Hamburg "Biophysical and Socio-economic Frame Conditions for the Sustainable Management of Natural Resources"

Identification of Root-knot Nematode Species Infecting Banana and Grape Orchards in Ismailia Governorate, Egypt

¹Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Institute for National and International Plant Health, Germany
²Suez Canal University, Department of Agricultural Botany, Egypt
³Suez Canal University, Department of Plant Protection, Egypt

Abstract

Samples of banana and grape roots infected with root-knot nematode, Meloidogyne spp., were collected from three different regions viz. Abou-Khalifa, Abou-Swair regions and Faculty of Agriculture Experimental Farm; representing Ismailia governorate, Egypt; and extracted females were used to identify detected root-knot nematode populations by using perineal patterns and SCAR-PCR techniques. Examination of the perineal patterns of the root-knot nematode females revealed the presence of three different species of Meloidogyne. Four root-knot nematode populations were identified as M. incognita from banana roots in Abou-Khalifa region, from grape roots in Abou-Swair region and from banana and grape roots in Faculty of Agriculture Experimental Farm. One root-knot nematode population was identified as M. arenaria from grape roots in Abou-Khalifa region, and one root-knot nematode population was identified as M. javanica from grape roots in Abou-Swair region. Using Sequence Characterized Amplified Region (SCAR) based PCR assays and DNA Gel Documentation System (D.G.D.S) programme analysis to identify the detected root-knot nematode populations, the same results were obtained and the species M. incognita, M. arenaria and M. javanica were easily differentiated. A 1200 bp fragment was detected in four root-knot nematode populations from banana roots in Abou-Khalifa region, grape roots in Abou-Swair region and from banana and grape roots in Faculty of Agriculture Experimental Farm. These populations were identified as M. incognita. A 420 bp fragment was detected in one root-knot nematode population identified as M. arenaria from grape roots in Abou-Khalifa region. In addition a 670 bp fragment was detected in one root-knot nematode population identified as M. javanica from grape roots in Abou-Swair region.

Keywords: Meloidogyne spp, root-knot nematode

Contact Address: Mohamed Baklawa, Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Institute for National and International Plant Health, Messeweg 11/12, 38104 Braunschweig, Germany, e-mail: mohamedbaklawayahoo.com