Effects of Different Trichoderma harzianum Strains on Growth and Production of Secondary Metabolites in Trigonella foenum-graecum
Zahra Sadat Hosseini1, Majid Majidian2, Tahereh Hasanloo1, Mozhgan Kowsari1
1Agricultural Biotechnology Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Iran
Trigonellin is one of the most potent antidiabetic so far extracted from plant sources. Influence of two Trichoderma harzianum isolates (chit4215MK and T8-7MK) on trigonellin production and growth factors of two Trigonella foenum-graecum ecotypes (Hamedan and Bandarabbas) were investigated under greenhouse conditions. The results showed that the two ecotypes are different in terms of growth factors. So, Hamadan ecotype showed longer stem length and Bandarabbas ecotype showed higher number of lateral branches and pod numbers. It was observed that Trichoderma strains have different effects on the growth of Hamadan ecotypes stem and T8-7MK strain was better than chit4215MK. However, no shoot length differences were observed between the control and Trichoderma treated plants for the Bandarabbas ecotype. The data revealed that, there was no significant difference between ecotypes, treatments and the interaction effects of treatment and ecotypes for peroxidase activity and total soluble carbohydrate content. A significantly higher content of trigonellin (4 mg g-1 DW) was achieved in T8-7MK Trichoderma strain treated Bandarabbas ecotype that was 1.6-fold higher than the control plants. Interestingly, a significantly higher content of trigonellin (3.5 mg g-1 DW) was obtained in chit4215MK Trichoderma strain treated Hamedan ecotype that was 1.3- fold higher than the control plants. The content of trigonellin in treated Bandarabbas ecotype was higher than the treated Hamedan ecotype (1.3 times more). It was showed that treatment with Trichoderma can be able to regulate trigonellin biosynthes and different strains have different effects on different fenugreek ecotypes.
Keywords: Fenugreek, growth, secondary metabolites, Trichoderma harzianum
Contact Address: Majid Majidian, University of Guilan, Dept. of Agronomy and Plant Breeding, 41635-1314 Rasht, Iran, e-mail: ma_majidianguilan.ac.ir