Applying in vitro Gas Production Technique to Assess the Nutritive Value of Gmelina arborea Leaves
Oluwanike Abiola-Olagunju1, Victor Akinwande2, Adejoke Mako2, Gbenga A. Adedokun1
1Lead City University, Dept. of Microbiology, Nigeria
In vitro gas fermentation method was used to evaluate the nutritive value of different forms of Gmelina arborea leaves. Two experiments were conducted. Experiment 1 involved the determination of chemical composition of green, yellow and brown leaves. In experiment 2 , the nutritive value of green, yellow and brown leaves of Gmelina arborea was assessed by in vitro gas production technique for total gas production over a period of 24 hr and to predict organic matter digestibility (OMD), metabolisable energy (ME) and short chain fatty acid (SCFA). Methane volume (CH4) was measures after incubation at 24 hr. The in vitro gas production (IVGP) characteristics (insoluble degradable fraction (b), potential degradability (a+b), rate of degradation (c) and effective degradation (ED)) were also predicted.
Keywords: Chemical composition, Gmelina arborea leaves, in vitro gas production, nutritive value
Contact Address: Oluwanike Abiola-Olagunju, Lead City University, Dept. of Microbiology, Toll Gate Area, Ibadan, Nigeria, e-mail: nikkymakky07yahoo.com