NURHAYATI, GRETE THINGGAARD, UDO TER MEULEN
University of Jambi, Department of Animal Nutrition and Feed Science, Indonesia
Georg-August-Universität Göttingen, Institute of Animal Physiology and Nutrition, Germany
An experiment was conducted to evaluate the accumulation of dietary N-CCC in yolks and albumen of hens fed diets containing N-CCC during egg development. Twenty 280 days old Brown breed layer hens were fed a N-CCC free balanced diet with 16.37% crude protein and 11.48 MJ ME/kg. In completely randomised design, the hens were divided into 4 groups (A,B,C and D) and given on one of the 4 treatments: 0 mg N-CCC/kg feed (control diet/group A), 5 mg N-CCC/kg feed (group B), 50 mg N-CCC/kg feed (group C) and 100 ppm mg N-CCC/kg feed (group D) for 11 days. During the 7 days followed, N-CCC treatments were withdrawn and all chickens restored to feeding on the control diet. Eggs were collected daily during both periods and egg yolks and albumen were separated. The N content was measured using a coupled Elemental Analyser-Continuous Flow II Interface-Isotope Ratio Mass Spectrometer (EA-ConFloII Interface-IRMS) and the N excess ( N-ex) and atom percentage N (At%) calculated. There was no significant () difference in N-ex and At% of egg yolks and albumen of group B both during 11 days of feeding on N-CCC containing diets and during the 7 days N" CCC diets withdrawal. Feeding with N" CCC affected () N-ex and At% in egg yolks and albumen of group C and D from 8 and 3 days respectively after the beginning of feeding N-CCC diets, and up to 2 and 4 days after N" CCC diets were withdrawn, respectively. The N-ex and At% in egg yolks tended to be higher than in albumen during the treatment period. The N concentrations reduced after N-CCC diets were withdrawn. These results suggest that hens might transfer excess dietary N-CCC or its metabolites into eggs and N accumulates during egg development. However, by this method it is not possible to determine if N is still bound in CCC or in its metabolite products.
Keywords: Atom percentage N, chlorocholine chloride, N, egg